	   SnapGene      tGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCgccaccatgaccatggaatctggagccgagaaccagcagagtggagatgcagctgtaacagaagctgaaaaccaacaaatgacagttcaagcccagccacagattgccacattagcccaggtatctatgccagcagctcatgcaacatcatctgctcccaccgtaactctagtacagctgcccaatgggcagacagttcaagtccatggagtcattcaggcggcccagccatcagttattcagtctccacaagtccaaacagttcagatttcaactattgcagaaagtgaagattcacaggagtcagtggatagtgtaactgattcccaaaagcgaagggaaattctttcaaggaggccttcctacaggaaaattttgaatgacttatcttctgatgcaccaggagtgccaaggattgaagaagagaagtctgaagaggagacttcagcacctgccatcaccactgtaacggtgccaactccaatttaccaaactagcagtggacagtatattgccattacccagggaggagcaatacagctggctaacaatggtaccgatggggtacagggcctgcaaacattaaccatgaccaatgcagcagccactcagccgggtactaccattctacagtatgcacagaccactgatggacagcagatcttagtgcccagcaaccaagttgttgttcaagctgcctctggagacgtacaaacataccagattcgcacagcacccactagcactattgcccctggagttgttatggcatcctccccagcacttcctacacagcctgctgaagaagcagcacgaaagagagaggtccgtctaatgaagaacagggaagcagctcgagagtgtcgtagaaagaagaaagaatatgtgaaatgtttagaaaacagagtggcagtgcttgaaaatcaaaacaagacattgattgaggagctaaaagcacttaaggacctttactgccacaaatcagatCTCGAGTACCCATACGACGTACCTGATTATGCAACTTATCCTTATGATGTACCTGATTATGCTAGCTATCCTTATGATGTCCCAGACTACGCATAGTGAGGGCCCGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG  <é                  !                      #      +                   (                                     #                                  $                         !   -            /      "   /      )      -            #                               +   $               !   !   9         #                        !                        "               "   $                                                         %      &            %                     &   -      	                              !         	               "                     +   (   !      !   +                   #         #                     $            "                                 &   1   $          3      #   "               !               $   $      -                     *   !      #      &            0   +   !   $   %   %      $         1      -         Y áìþf�_	`	Ñ	
X
}
†
‡
+UýÀý�ŸŽB~� á xâ bÇ�Éa	üžÜáõd‚ß�Œg7ÖAÂ Z ÷ í!µ	¼	Ò	9
ˆ
±
9kXSþ CèV‘  ÈNuôùÿgtH	ß	
F
Y
~
,~V²ñþÁšP"Ô;— t û cÐåŠæMpÈËy¯k·b	x	§u�Ÿ�8Ã! ´É*A‘Y	t	„	ˆ	ÏG^¦Ý“)W‰àš¿E�ÂS“×p÷  ÎãžïWU¾<	ý´ÆÌç�¨ù<pƒ�rK"hƒB�i½ â y!°F}aÊò½Žâ±\höe'– ø ¬_±‘Ø†¶	:
²
·
lY¡z&T¡¼e{õøé Œ MR’2ãî"1‰
´`u¯DZ¡Îï/ ´Õ¤úÓ	º
:r’ó^ÿLºÿ}øDy¸W’ [ fó½	Ø	æšKKÂDoË¨†Ø!#  ÉOV•Ó>Z
 
®
W³òAäôY�“@#YrØ8•bT 6€ ]‡ºvºGË u™	ä	]
-Ov¾”ÿ7Ýê¾ûJ+ œ {Ù„hm¦	e	à	G

j²-xŠ/Hœ¥ç3DWÂÝOt›QlSæÕ‰<q4O    “ T˜õú¿ã		I	
@
a
JP„û©0K¯ºÌ~H˜£ dnÉñi÷µc	HNsv‚Ê
’÷Xè9 Öæbqzíïy	ú	À"+uaŸÄ u ü á‹çN°C™¸7	¨ç�«nÉÁßWû'( t˜ÑÌl ` ƒ¸Šñ:„¯\§÷Tœìe3( ÷Û	\_dkÜßÿ	#’u	…	=
zÂk×Þõ”µ‡ŠFŽv“²+¤”£Ø™øIc  ýÕ+é&2<Q%Z	m	_§$r*H`á›ÃXqƒ €¦ µÊ4*¨	:H‚–Xüˆ(uf ØB‰	ÐÞ†ÐŸÀáTY  ¯èDéCÃáoÇä87�©qç«ò % ÏðÿVï	‹~‘1s)2YCj 7 ä ÅÞüX�e®Ì)¿@fóþ%Úè=Ã-3Liwx ŸN“=	p
µºÍR£¬út„Ø#.6„�ùN`Ž�¡Å¾ÆÎ ¹ Å î AJûE|¥ë˜Ë4	–	�¡�¯Ë²ñäfôI´º 1 z±G_b{{	¤	Â(i#$—ÐX > ã zÚ"8sê- ¾1ãE÷=()ôÜ   « pÃ	<~óü	É
Wmqg]Ÿ.bóc#T# ù Ì`¯²ãí®ES½ÑÖ„µ
¸
|»E\¤£'IWý“y|öQ¦ ­Ù0õ#;
Ç
mq|^­¥æòùê #Y­ôÙâ‡²·	³
LNZ¢AU\”½Ù$ª P’UKÛF{Åø«¢ëQÛfOmd¹«- S›
yn¬¿_Ïð&å* M � Õ N2ñ]öŒC–vÅ EÃáœã® È þ %•3µ32l€™Š
}Å¦(µ$a°[›¢¹0y  3j‹¾[“fäï#›¨Æ‰ýä  . dg²@¥ºÄûq‰´¹	¤ÎqMúÄÒËÐj’ )œ%   C ~ ¥µ»
T»f~¹bÔðYz¬¹Xý  µÖ‚7äÁ	
“Ñ]§ UÛ;ù§÷+â$ c w Ì ~ÀjÅ	Ô	5
;s°ÅôC_¶Õé ’O]¸ÆêpèEw§“ /Œ9	¾	ì	U
  +ÜèLÃ!(·Ù" \ ¼ ñ D›þ�éÇP–ÞLhÑLp•×	9 íg­gø«!	ç› EÌþÅ^ ¨„·ôä›ÎÛÙ	ªc«ëvz©‡  ²ÓP–?Çð 		Ï	[
ž
¬
¯
šÚ?BZ‘ó
W^scówg  8óp�õ”ZV÷_U!�1  , | ÊW€Ô	
Å
´oAÂÐÙ9‘7à!  Å�	u
”
¡
ª
À
Ú
â
PX˜½ÇåçË}Ç$ES–ú¨žûL˜/- ˆ»qÞë?wÒ«KâHL
^
m
w
Â
sw»¿EðR‹•¿ È®—8;Q	 §¿V HHÎú\´Ìîš	�šÉâ‘:Ç©´ÞT�®ýoîü5K ^»ð£
.†ŠÎÒÖHzºéÍÍäò�ôùÇÍLø_ Gz7’—¡ÇåuÝv±	å	Pì …ÝëÂ»š/  � îúkÖpÙ[¨è[^n•§	á	z
".0I¦Âè4EP„œmŒRñBTÕ©ÑÖ÷¯ìQ™% ¡|ÚnÒÖNsÖf	‘	Í
b�ëXau6±hŠÛ@"  ŽM^y…'ñ
Hy‹¢ÿN4Œ£­R±<Ô.k˜Ý=5êPl/ všBTCiª	H
€
è
!k³˜3ÇÃÞ½mÉ&6Ggç
IU£ÊªÒLjr$?~,fí ÓK
	J	KQü;>ú—Í1Lº<i†Íh�™Ó¤õ) ! Ú .»$;žR€”¼ˆ9·
O
g
Ö
€ªÉÙ#í°»1>µŒìÜN­ ˜ YösÕfNR®		'	M	U	TZ…ÒÜ?›¤~üº- j ” ¤ ² 7Ue=-n™W©ûÀWä-	
A
b
–
Ü
ä
†šðü&Z}_í™ÐìïI2 à ÆóøeÞ	E
…
8jûWTà¿�f:–U� å)LVoÊj|¶w	‡	tÅË�§øg™¾&J•!‚Žö e1ò òK®ÁY #  U”Êd	?
`
iu±½IOwƒ‰“Üãq®¹Ëå7aéSúIN sàamìîðBG¿	·‰9SmÈÞ ×öÔ^cÞ"%);§	yÁj#‡ž’*¢˜Xe $ çÄÝMŒ’(o
ã,0-v�(wæ Å yr;{^é3	z	£	û	ŽÁ'l#b "¬âØìóO­TDJôE{ºp÷“¬xPåN¥©cª, L Ô ý 2iŠ½e´2š|Äm'š¸Âàä›üã)  B b v }¤¿c±¹4
Sè‘ù·o¸Ê¦ÓçïöXŸ(¦ü šì§ƒŒ¬¶èf8	©b•ªÝêÛÄ Òï�
©
Ä
Ù
á
O—Ùo€nÊò|]�Uòö�vßf+ pÝ>yªÍJ[– Æáätl
r…™ºÍá„¹ÆÌãŒ¨³ø:P€ÁUÌíK4$ u�™íùAjÕomÕ”y
 !a—¡Á‹°5;Q°ðfHÐÑÚ>}? i Ò,�ÔN
–Ì…šî=´‹� 
U{v	†	>
úßâJp­¸Ê” e�•R�ùHM Ü]`lÝë !$(]'¢	¾âk¶ˆ�/"Ç),åd !1ë‚µ×eb3
a{©Ãlö&’w¥·¤ãšžb©! oŒønà
s“¨
„Ì–Øn•Åâ‹™G�Ï³ÙuJ >! Öê 'r&¡	`ƒ¨Ë•%IÄá‘˜oœ¬·É.Æ€¶ta¨9 *þ,=RXqŸ	 	¬	]^_-.“”+«¶ÇÈ ÄÅY~žŸqrs`r„ðñþ	
�‚§7 � P+J-3S��&/,ÀÔÝa™ ¬ÿ!ƒ‹8 	†ÿ»¥üƒ>Y[	n	­	Ë	`ÊsâàäZÅ†s…ò‡# � Â ô GQÅ,Kr5àü4cIƒ<-—·¨ù ¡ÚV "v I ¿ Ý Ð+~	œ	©	
·ÁÉÞxƒó<F)@„Œ S ¶.�~0;?‘Ù5Õbs¾šÌË9[ O ËTŽ
žê'+YEý‰­±áèg ¨ × ŽÎ\	®	Ì	R
5”UÿKt 4óJ¼Ýý„?Zo	ÑÔ*ß$t} GáóˆE … 
7‡_Ëø—¦¶:Ci	aËö‡Ñ¢	´ƒ[Æ,°Z†Á Ù ^ðŠ	ÛœäãÁâå‡°! r ‘ õ RvªÆLÇsˆáêD¸‚¤p+å1‘
$.˜¸I2¶¼Ö Ã HnÁ	6ýÁß³”	ÈØ„ª	Fr©ús¢¿W^ca  Ê -.5Äâ>QJ£]h¬#w ,c°é9FE5²ÐdqˆÇÐC98=ëŠ¡ÛÚèó J g Ò Þ Ðñòj
Œ¸èlÊOãôS�ú ƒ À m›�	ª	É	øµÂß�’,*„ÉD… & n p *k·, ¨»½Ao ( Ñ‡¡W	ð	
J&€y2t3Z=G*0k" 4 ; ¶ ë >øB!ÏY¹¦bf*v6
Ûéî�«c¿ÍmF[\ 8 F T _ `—¤)¿ ¯Í¥
g<’Ú»öMx›ÉC  åÆ/ž‹Aôÿ&16?®.Öæ�jyNq{ž: ·�¨ßý+Ž4Àë@ec>Ä4ôt:Ì žH(ƒÎ, w|B·ÏÙZþ.�¿" P 'ÂM¡ƒU�n».¤¼!d&ÏFcŠû{®â}O¯jR$ �Ì5P=O”>	q
áŒÔ¶­û…²¤$7…²ñúD‘ÆÏÇéh  ½»ÙŸëŒ
0 ª*Sui�Ù/‘¶Àaß¢Ï  W Š Kß]�<]	r	—	³	M�°ò²{‡@5?» © ç£5	�­³å)‚õß'V0L º Æ ï B“ü}¦Ï™ÌÙS
	w¢®Ì‡` uµ * Ø 5FîLYì¯	Í	
’
¾
6îLg•§Jh" ôh{½ã'²Óñ09H”Ò@c¥	Ã%…$Hk˜Ñô 5³Þ�[‹||	Ò+à")¾ÒÛq{þâ‰æCFY 2 � l�À'Kþ…`uj~¹¡\Qmœ Ðp	Ð
Õy%� ù‰@….ß÷xj	ÌˆÔäÒFïÍ:4AÄÔõÂ ? ¤Ì§%�£ë;2)	„�- † ä {ƒ#ˆ9tÉw·!Ò
b£*Ç±[Ö Û8`˜Dç	¿×÷‹ø
µ>k\²ÿ‡µ,2Ý ¡ •Zæ�	÷	Ê
n^ 9(Âoôé\n$I–U §¾ÄÚI†¢
(=_‰	�åhk4æ×AˆÜ q|ªÈºôRr[/äv¼‹í° ¬ óÂ™�ñ‹	ò	ý	'
,
XÜx�cãd±½ ö wÇMatëýsG	»	
|
}ðR™üOž!Óç% s ú Í³ÈÏä‰î¯Ex®T`½X	¦¼ÎF]¥€æ’°(;oŽDRo «‘°â0£×ù…¶
¹
q%¹þJCz Íî÷nÊ§.·÷×& ’ › S\†¹zØ¹Fƒ¾Ò¥ã	,¤¨æ2/JXs�?×”6}G3¢½ hö´_rÉðª®¦tÀçúžëd' üÚ3[jÛþ1P$l	<
Ý�…Ö†G_ûu±£'Htb 6 ®ûCBd­ÂËàenŠò$¹Ù76Q«}s×5X_Äñ% 0 = Ÿ ¸ Ä í @IoÙúDÂ
7,êz—•	È
 0žã
<óHÏó³S ®/"R¼Úá±´
DKM[£ÄHVü[]¤ØÚlñ  $Zðõ‹Åk˜š
¥#•«¾^%­x Ë fi´6?ãpˆ³¸	Ä	ÄBÔ¦Te\ Ïi«›$ .ãªÆ‹ë	OKguÐŸçyý”Ö]  ±Ä	t
¼>ÁÆDVRù§ú. êGÑFù6³Ü€‰ÈÑD�ëŠ9G­ñóÜS­nÆº^&  SØLÑB]rx§ç]©Õ	G2Æêƒ¬‹Ô¢g®ÜPé+eìk- -d#:mQ“»Vr¨‡e8QV¶		f
Õ
Y:|ù^ì"£ÏìgÒÛ1¬ô  ß T„
7iVˆä­À`Žo	 :�8‘Ž'Ý~ A K a Ó ™¦dòSóDz¹”Üéoøæõû+ h Ñ~+©ŸÔk
œ
à
mË4POÐñ|T n‹z§
ù!dâ�äÿ= Ž O…pœž	«	Ê	%Ãà�—Æ-˜�«µÄ…_† H N Á ûb¶>4¶D§½øwàËòÊE „ Ö 3ò^÷�Ü9h	)4õ
³“F‚+¯ q É +bu84·-±ÀÏÞ=P�²“	~ÆBE\¾1»Õ ' o jl¶š÷§´%+º¼ : E ÿ =–£§Î¸¥3u5ê>dí€ªœÈBEZpz! &4<ºØm‹
à©Ÿ) c¶Øhb€± -£zð|µ®Q  ) V ‰ 4’\¢íKXØ
½
LK±�z†?/4> € kŒ¿g&Òð/€ÏÏ
½jŒ[låB	 ‚$„ŠÓþÕ+1 ¦”¡Áå'ˆœ©Ç€	ñ	Zw'3nu»Ö@[H• …¸�ürF	º	
¥ïrŽN> ŸÌmÉ-¡n / 5 < · ì ?ØùC2ZOcg+dŸñÜPâtÖ4�&GsR 
eh³!j—»ÅŠÃ	Ã¤fÏž¥ûdÿÎk“\" 
 "Ðº§Aw¦æ7UµÔ
�ê»‚¬ÀÅÓÑù*0]  ` ˜*R96ChU“ÛN{}n G „Šy8a¦
µ(3=¼÷c—œªßÊñ^þ< 9 D at¦;¶°¹¿ÎÝ±ö=A©³gÿ,y{´­ºDPY U  ˆ ¥ ÀƒJ¼
K°¼mºÕ?ZÒ”Ô 	 ·×	1 iåE	Â	žð~Žežþ3lø,r ƒŸ8‚I`°5BJ´õ'2<@¯¨–©z³]ÓO|;+  ¶0HŒ
”•ï 789:;{|}�¯cd6^çèéêÜÝúûüý:k•¨ìÒ:;( æ }æ%Ç‡�#)Ÿ
 
í
ó
 –	°Ò7/_×ëVÖÞ<�š;íØã<Ÿ!  ¸�$à$	*	Õ	6
î
—
±ÓdÖÅ0T–P‰¹Ç¿éƒ{¨ d ‘©ÁÈàvkˆÆ	t?Øìëˆ7;ÉÙ=! @,5ŽÁÒ
ô
±ìA õ`8ÕßêíB“õ‚›q<Íîxä+ x Í ç ~ç&þ"Ë{*€ ÷û	
!
÷
ü
<ÆfD·¥5`Wu×*0ß=^F6”  œk�á:	Ö	7
V
)ä²-�UÝÿIQŠºÀ„|À —í	UÉe}¡C1,©¸Ú 0¿	Ï¡þ©[éMOÄ"&)�·  Ÿ¹�%I¬%	+	J
ï
„˜ÔxÅ×¸ÆÐÚ—4@È/ê#  ’¢ªÂêw�È1	Q—ßM¥½ed‹µìNãô~<–ÊSá# Q ½ ò EÃÿ€ÉlVèÙ�×¼iàmž'|¯P:‹> ] e (œDNŽo‰FÇ	/"@ˆGMq	8´Ø
hk.a Ýá„uÒ'ÐÙío·Ë8iü¥^‰l °Ú# xÍQAhvÂÓ	é	r
BW]·5®9îú!¥¬FÍûEïåêï	i �h6b-ù‚
#èÖCö2=³ÿÆ >P6®	"	
õ
²Áíaœàë”(rÔ8†Ð.y’ÇÐ4 î®•�¬?	â�Õ!öü†Wƒœ³%òÎ‰_Èß y :õ�	=ù#øEwTaj+é_{Ú·A‹à• è èð'¡©…°¸š¡ì«Çg«+6yvØ‚àÌt½Á£ #¾Ì¼ÚE‘” œÏø
ê
ý
¸¦vX1Y0’ª€ˆ7$ Î VÿÁå|+1ÜüA	P	Ú	"
�
ø
1dm¬µìš¿>K…Gb¡ÊÐ  X à ŽÈ^	´	Ð	8
W
°
*�±Ûüôc€Þ‹A6Õ@}Á �@ñ;	s	ƒ	³Ž[VˆqÁ’h¼ ‹ L³ÔQ^�×	‘å]y™³J_t|»dôCx…" —=lâ			˜	\
Ÿ
­
Nú.G›@CVóN’k RXˆÜp5 —Õ˜6	ù	M‘!~‚öæ*ƒ[öW`›V&2 è9ôq´Ï•àW‚¥  èþî	?¢Â2ø1M�ªÍ ª ¤Vp®ÊfðD-"aò("¹ÛW ËXÐÕƒÆ
@¢{ªêP’#¸ Ç ”1\~§xˆBÄâ  - } �ÃÀ	

£¯ÐµÍp\ÃNÑÚ:Åéa*‘vá » ð Cý÷šÍÚ 	T
ÿ* '8¶  + { Æÿ	Ž	Î	
“
«
¿
7™Ææ~	Ï^K—0 G‘ïí°	K
v
¢
Á
ÕïQ¾yèÇÌ–Ÿü¦™÷&   MZ&Z�	Ì
ç
ð
þM3`¢¼lÈÓ%-5AF	Tj—¨Éö©ëKi#˜$1 — £ ± Ù 6X<ºJM­&	,	L	T	
•
Û
ã
…™ïû%S=YÈØ¹ÑÛ;>h˜ë}û0¹ëM$ 0“ä(KÅòÉdÝ	D
_
htŸ°¼ÄñSŒ¦¾ÛfŒ˜½%6IõT  # rßõxÃÓ:L‹A‘è2	¦	n
xÀFi&†Rlv¡—W h‰¼«|£«¾ë¬³1IRÚ¶™·OÒäMÃî¨¥â3 Üì=@iœxÔIZ•ÅÓãîM
x
Ã
Ø
q˜¹àN–ÀËÉ{¯ïe²í9<
õŠŽ=ÀËÞ  0ê�´Tam\2
½ájmáÎ(QŒGL# )Iº¤û‚WŒ\É,‚Ê’¿ÓÜnªµÿßã}pïýŠ6" R ¾ Ü ó FÄ qœÏß	}é}	›	

�Ø*;ÈrŸ°ÙU‚;?çZ § 6IÊ–]µÍïQ
ÓÚ›ã#¡|¯þJÀD m©ÀÆE‡p£‡Ï*×0ê	#‰H¢r]gµÙb` f m ‚ Ñ )† ñÈ	i
çIkNŽâÈR/nù 3 ^ µ ê _÷A(�¾ÿ*aŠ
¤
­båóºõ
9lM�! Ž�ÁLO ‚¼G/‚‹Óÿ-v{»AÎ%Îú
C¿ÞiÎ Þæ;q	²	‘
v­í¬†(¦Þ&_g! &²â8“ÑŠ!ÑÚpxz¸„ýP› £ˆ?œ¢ÈvÞöwæ	Ñ
ÖÓîÌ9j3@Ž±Ã´ ò½YH{…˜¹ö	&
+
Qj8œèŠìm¼Û¯$ š [yÎ×w­/¢ø_¼W	â	{
Í§å¯:Qûn��ÖCÒ5BíFöæ$ ž ûúBiAÚ¬Êéy–k	‰#/^¸6€Õ5…�ï;ò°GÎÃ²ð& ïŠ©ào°ê	™
CJtÃ
ÓG"SZæ[xª×üÕðš-  Rl~øq×†\P\¨ÃÔ			e
s
X1‰éF]!ðÎòCUfø¦­R¬Åë
j @ }¥c¨žÓƒ
ß
‡÷37��Ü Ûúog	$ô	3¦²�´ <¢i7Í¤×<Ot4’	ßécžìb×D*
ï ÇÔ �‘%&.Îì×Î
Yvi…‹>.A „�N–TÓ
cÂ£ ºO��á¤=sÕÈ/Q ‡ s‰:Qx¸¾Ü7²lþ+Þ>Ñ“m* å |$�G_h†¯"(žD	
ì
ò
ÿ
4I�³îzŒb5®Õ€™29”²ë\Ñ×9!  !?uÊzÑßöú	#	)	
ö
û
¤ÔÞìAS•)/ˆ¾‡6‚zÈ5 ›–j«I
TãÈŒÄý¨3?HN%¶# ÜCEç0	ØŽÖß‡n¶Ê7hl�¤´&M]ók³ gŠ-`à wau­		è	�
"ÀV\4ù1'V«ÓÏ-ˆ3Þî& ï9U¯™ÀD�“0@	O	é
l´ø"™÷¾xèJX„Ës¼@ŠÉ 	‚	œùF\xûU˜Mbj‡pÛo‘ÔB4 –Ôçýø	>Lo ÎïÁ!`ñšŒ%¤ÌV [ö~Â‚?z)Ãèá0 z – ¢ ° W;LYþ	K	S	�	
Ë
æ
îú$Rý<2_¡»:=kÒ,Îêi|õê]¸¥J—–ö+ " gªÛ/<›JÄYŠÄçíÜ	C
×
gpž¯¸hÊÚzÙî±¼5¶Lu‰�Âí¤Ý! ¦ Û /é(p¹S•›£`	
1
P
[Èl ÍØI¿$ l ´ Ð é ¨ö@¿Å†Ÿ�½)Ÿ¢ 
h
�æþ)¬,a$áB¾	fÝ% ñ¢¼%X±‡>›‰õ
:¸õ	%
*
�
¬«i7›wƒ2?çfO³®% ™ Z}÷¬Ö«É¡O¯»	V	d
˜
ˆs	\ YœåñBZew«± ;€$Í3Þ
Þó†¥€ŒîÓ$ r €½ÛFg•S�ÐÞõù(	C	
ë
ú
þ
3œ¹ÓÝ@‡ý½1“�8 t¿’/æ	/	N	SU[§w02M»‰ • ¯ Vü}ÁXýR	Œ	
å
í›[îyÀÑ çð3£Ë1 k ¥ ³ Ï fõ?.Woš ÃXšÃžæôÛ	ô	ÿ	$
)
0
B
�
Zfo®·ý(`šŸKeHe²¾ :«.2Ýå.	B	c
—
Ý
ù
2‡›Ýò~ðALd†Ò- ­ ® 89ô>ªÂòó,-Q	ó	þ	#
(
-
.
/
Ž
Yen­¶ñ'žíîï?@äåæJcd¢Ñ  0Ä  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                      .                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         	                                                                                                                                                                                                                                                                     +   	                                                 	                                                                     	                                   	                      
                  	            9               
                                                                                                            0          $   !                   ƒ   ]         j   b               [                                                                                                                                                                           .                                                                                                                                                                                                                                                                                             	                                                                                                 + 	                 	                       	             	        
      	    9     
                                    0    $ !       ƒ ]   j b     [   vQ  w{  w�  wÇ  |+  �i  ‰C¡mõvk ÂõX�ù	V	Û	þ	0
1
P
—
Ý$?_z±Ãi€`w¿§õxò_
<Ë:qÞ"®ó” Un�°NñÇÇ§?^
�É ” .U²Ê$?nÙÔNR®
		‘	^
w
¡
£
Í
è
�ÉüKQI�´?EºÞ›Ç^Ð�ª¿ÛÄ•Gbc˜G"G˜GGµ©À	µg¯¼Ø	T¨Ÿ¼£@
“º£¬¾ÔÊ‚¶{l&µ‡[�ó|ø`
¬µœ�:	²·‹+„ÅÃÛ	B
a|ö³ƒ¹ î Aû˜Ë´$+·Ùt
¼ú|[ð’ãü’‘ãOWz¡êæ
»kñ\eÿtú¨/J ¢µ-/Ô8iÚÕ…°uÈ¹ î Aû˜ËWf6´‰x* ¡? ¤t˜ÛÅƒÛd¹ÛÛ¹–›ùF <»öÉ†|ÒUùÛt{Û¹¨žt˜ùgF
~
Á(¨N¶W!	†ÎéŠ
(Uri¹fK%.™
‰ªiÙ
á
W#•M’o
Å’žÐ‘ãùˆ	Ø	
:Oj²Wz¡¿æêHqD’Îw­�xsß&Š�ú	a’v†¨Å.†SÃÄ«:‚¢þÛ	Ü	B
C
f®)L†›ûœAò�	woA:T

	—LinånÉiµs�úšXÃo·z
PÑúJò�"z¨Å†¢) t˜UùÛgtF
~
{ÅƒÁÛd¹qa¡$¨Å†S¢)†	'3_kµ‡” U|;ÖöÕÒNWª	
	á	
H
^
Ö
ä
s†»Îì¦�ÃÉåéLiÐíZ°ÕŒ1¨Å†:‚¢þ)†Îé¡’žM’<	o
´ùƒ"-ƒ�� Å �G‚vÎ«H§îÌÃ›þƒ–©]>Y:ù#é{·t˜ÿÎiw­ÚÕ…�°uxsÈÜ=Iq¹9
×\	­µˆÐ). +·ÙŒ. Y®Ûsª»$+ÛÃ¹·Ù Ív•g¼i¥®9e¿à'ÝŠrÔ'qtaÀÊZ* _9	¤
	LiìiÔYîþx
Àïõê—ÓK™j–‰ìiÔx
Àï+·Ù‰x–ß&Š@¡m#TÖ	äI„iŠ½eüãzØ¹
s¹·ž<	´Ìùƒ"ƒ�½ŸRtØ.�`¡ÎÈ2ZkOÜt&=Gs™ Z/JÄ¬¡»V	Ü	C
gž¯Úœ¼5Bí­«^qzy	ú	Àa ë?kpâ[[§	L
w
Â
0•¿èEÈ®ñBT÷8;	Q¿ “ T˜ôó}ùú¿ãòH	Ø	
@
a
~\H—¨†›œ[ûbÔ3¬:
løé„‰lÚó}òØ	\¨†ë?âL
w
Â
•¿È®8;	¿ kp[[§	0èEñBT÷QŸ��þ,$B¾ÝéCoä�<—	Mò‡> ë?k–pâ[K[§ 			J	L
[
w
ž
¬
Â
Qü0;•š¿è?BE1‘ºÈ<®ñBTWÍh÷8;	Q¤¿õ™ Z/JÄ¬¡»V	Ü	C
gž¯›œ[ûÚbœ¼5BÔ3».ŠÒzºÍÍùÍ¯èD87çò.÷Ìä4AõgªÙ¶LÂí¤Æ�¨p¼/ÿ
¿Þ0	´Móà^ï&á x ÉáõdäÛÙ	c«ëŠ K]Z{½‰�²0ß�ÒF²({Â˜ÑÔQ0	^ï´Móà&m«ë¬IOäM¨ˆ?ÞöwÓîÌ93@ÃEëfI	7• ¯ VXýR	Œ	
å
íÑÇÏ	¯
Úó
ÚÊ‹Þçü�¿7	¨¾=Ã÷3Xü=ˆÜf¤T
®Êðÿ* '¶¹œÂAh�1	:	=]²·5�îU_ÀFÍ‹–ï ÐÞõù(	ú
ÓÝ@‡½�	u	…	=
Þ“”øTCè
k³˜½çIÊr?~ÐËkŸ~ Õ 2ñ]öŒ»
Ç
Ñ»E¹ÔYò®Û\_kÜÿ#µ‡+c>	"	
õ
ë”(†yÇ4‹ç7	¨Ú¾÷=ÜÇ
ÑòÊÞü�¿=Ã3Xüˆf–K 		J	[
ž
¬
Qü;š?B1‘º<WÍh¤õ«ëˆ¬?IÞöwÓîÌ93@OäM¨Ãý„Fú¢ad ˆÆ	?7r	»õ�à + { Æÿ	Ž	Î	
“
«
¿
7™Ææ~	Ï^K—0z – ¢ ° W;LYþ	K	S	�	
Ë
æ
îú$Rý<2_¡»:=kÒ,Îêi|õê]¸¥J—–ö²ÓP–?Çð 		Ï	[
ž
¬
¯
šÚ?BZ‘ó
W^scówg 3j‹¾[“fäï#›¨Æ‰ýäu�™íùAjÕomÕ”y
 !a—¡Á‹°5;Q°ðfHÐÑÚ>}? Å�	u
”
¡
ª
À
Ú
â
PX˜½ÇåçË}Ç$ES–ú¨žûL˜/@ }¥c¨žÓƒ
ß
‡÷37��ÜŸN“=	p
µºÍR£¬út„Ø#.6„�ùN`Ž�¡Å¾ÆÎ  “ T˜õú¿ã		I	
@
a
JP„û©0K¯ºÌ~H˜£	‚	œùF\xûU˜Mbj‡pÛo‘ÔB4m©ÀÆE‡p£‡Ï*×0ê	#‰H¢r]gµÙb`  i “ œ Tu{�™Òíù,Aj˜�ÕÙõoÔmÕú„¿hm”¦ã			I	e	à	
@
G
N
a
y

 !j²-JPax„Š—¡û/Hœ¥©Áç3DW–ÂÌÝ0KOt‹›Ql°5;Q…š°ðSf¯ºÌæHÐÕî=~‰´Ñ<Hq‹�˜Ú4>}£?O' f m o q ‚ É Ñ )+bmu©jlÀÆ8E‡¶4†·- ±ÀÏÞñ=Pp�š²£“	È	i
÷~‡ÆÏç*B§×0Iê	#kE‰´%HNŽ¢â+\rº¼¾1]gµ»ÈÙR/bnÕù`º Æ ï B“ü}¦Ï™ÌÙS
	w¢®Ì‡` uµêGÑFù6³Ü€‰ÈÑD�ëŠ9G­ñóÜS­nÆº^" ™ ZgªÛ/<›J}Ä÷YŠ¬ÄÖ«Éç¡O¯»í		V	‚	Ü	C
d
˜
×
ˆgpž¯¸hœùF\sxû	U\˜ÊÚM bjzY‡œÙåî±¼ñ5BZepw¶ÛLou‰�‘«ÂÔíB4¤Ý±š [yÎ0“ä(KÅ×òw­/¢Éø_d¼	W	‚	Ý	â	D
_
{
ÍhtŸ°¼œÄñùFS\xŒ§åûU˜¦¯¾Û:MQfûbjn�‡�Œ˜½Ö%6CpÒ5BIÛíFo‘ÔBö4æõTS ¶.�~0;?‘Ù5Õbs¾šÌË9[t¿’/æ	/	N	SU[§w02M»‰dnÉñi÷µc	HNsv‚Ê
’÷Xè9G „Šy8a¦
µ(3=¼÷c—œªßÊñ^þ<º Æ ï Bq“üeu�™íù1Aj|Õo}òm¦ªÈÕÏ”™ºÌÙôS
y
	 Rw¢®! a—¡òrÁÌ[‡‹K°/5;Q°äðfHv®ÁÐY¼Ñ`‹íÚ >}u°µ ?—í	UÉe}¡C1,©¸Ú  +Ö     ÿ  ³         ³)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄŸÙÇ'á¦5é¾˜b~c“›é†/Ñç6H@üfìuµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�\ C—…wuŸüÎaÃYUUÍÀŠÊŠÙ\À+ôî,gýñö€=g?2®¾«ýÿýJÀ…Ð;4õË ÃCøöMŒÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nåloê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+1X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»XíÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æí'[Žá�6JSÒn;øèˆ BÎHÌMV¥9×PÔ:hØQn:  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "Ã<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5677" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4886-5677" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5678-5746" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2585-3379" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="843-1431" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4127-4715" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1747-2175" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="13" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2189-2518" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2369-2504" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3555-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5747-5851" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1497-1545" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3773-3779" color="#ffffff" type="standard"/><Segment range="3780-3797" color="#ffffff" type="standard"/><Segment name="-35" range="3798-3803" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3818-3839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3725-3741" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3749-3765" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="733-759" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="763-789" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="793-819" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>133751de-e061-46cb-a9a0-62e6e87ef9e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="6:25:58">2023.6.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  5        ¶         ¶)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄUyfÑ�tW–ÒQ©U)jñ™!ñ›±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>p^ÜÖ|ó9‡eTtYW7(*+es ¬/Ð87¸±Ÿ÷ÄÚ õœüÊºú¯÷ýÿõ+?@ìÓ×,ãÙ4n3N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êíc·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜,{´�n;†<Ù)OI¸ïã¢"�8I#15Z”ç]CPè|u£aE¸é   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ! <?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1316" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="933" maxFusedRunOn="933" detectionMode="exactProteinMatch"><Segment range="1317-1328" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3752-3768" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3821-3842" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3776-3782" color="#ffffff" type="standard"/><Segment range="3783-3800" color="#ffffff" type="standard"/><Segment name="-35" range="3801-3806" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1500-1548" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5750-5854" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3558-3679" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2372-2507" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2192-2521" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1750-2178" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4130-4718" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-1434" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2588-3382" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5680" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4889-5680" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5681-5749" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="825" maxFusedRunOn="825" detectionMode="exactProteinMatch"><Segment range="733-822" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ˆ<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="R1" sequence="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite><BindingSite simplified="1" location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite simplified="1" location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite></Primer><Primer recentID="0" name="F1" sequence="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite><BindingSite simplified="1" location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite></Primer></Primers>
  ><Notes>
<UUID>554b079e-04cd-46e5-910a-3c8d9ddfc533</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:15:15">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *T      ú  ž         ž)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M¸92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄuGØp†™Q¶±çPaÆI!ñ›±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>p^ÜÖ|ó9‡eTtYW7(*+es ¬/Ð87¸±Ÿ÷ÄÚ õœüÊºú¯÷ýÿõ+?@ìÓ×,ãÙ4n3N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êíc·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=ørÇÀFïÆÿ˜,{´�n;†<Ù)OI¸ïã¢"�8I#15Z”ç]CPè|u£aE¸é   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !H<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5656" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4865-5656" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5657-5725" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2564-3358" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="822-1410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4106-4694" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1726-2154" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2168-2497" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2348-2483" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3534-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5726-5830" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="798" consecutiveTranslationNumbering="1" maxRunOn="801" maxFusedRunOn="801" detectionMode="exactProteinMatch"><Segment range="733-798" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1476-1524" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3752-3758" color="#ffffff" type="standard"/><Segment range="3759-3776" color="#ffffff" type="standard"/><Segment name="-35" range="3777-3782" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3797-3818" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3704-3720" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1292" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="909" maxFusedRunOn="909" detectionMode="exactProteinMatch"><Segment range="1293-1304" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>70bcc161-158a-4f5f-9630-d4c4e88666d6</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:9:43">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  
ˆý7zXZ  æÖ´F !   t/å£àaŠ
G] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x†÷|ÁP)9Õ}ž«*ÿi@G%]!Ž=±œ_\êÔ5MSï‡= ±3{‰ò’v•Â ÊÒ�ÙÃv”×¾NÌ›ÏÏ%û
!ÛA¬S­îo¼'¯±ŒJ<âÆ  R$á²€¡9O*Íˆíã'eRß®BbÙÎ£�¹|¯õšEçÆY+¿Mu†é’[Î’tå›|1ó»­”WáÅ[˜QŸ—ºhoX7Ù«ÙWùzi+Ö�ºo Ýš(6¿n‡rgHà¶oƒsµÑÚ¨PRøo¶.Iz}Ì²»C²¤ðQZøÅ²‡r“Q‡º3Üéßò8}ŒôMæD÷em…éŽÕ_ÓC„ž&ŒÅÃˆÍÆ)Á`k¸)r�Ø
<J‚p*±`Œ;À~˜îs¬GW®©bbŠQûZ³t1@ÅÝLb‹[ñW5vå»~T>Ê�P àþÚ¿^ÁDW~�D „ùÚÙ|(kÔ�9}Sfx�íZIaýöÍ;“«hü› ìÈ0H}ñéŠ3	óé)ç _H³]«;€FîÈÙ�È�sOaŽ :nnh¶�ŽäÕúÕ�Ÿ(³xüHBûŸSGá�)À„†Ùˆc†ùsÿcješw×Þ�mŠ´†Pë‡™ÿ¹§¸Û–ˆÜ}ÄÞFç.SMçÔ×*{xþÜ±>(3Os]à:ShhÙX½‚ô»‡£Ëdœþ�ã,´×\ð‘pÏ…DÈn­õÁæÐfâ6·2ÿÀsß²Y‡Sï£õ¥3
mF<z¯|?8]oý½2&�-<{ÔCm½^ƒ9´)ïÛçZlT \'‡€¥±˜êe§³�áór“>N—p|æ°–§Æ¢‰ ó¬¾¦EÊŒNliç³ÖóoÉs`o â0Wd%} ãÃP	8;Ã~QO¹Üs¶w•%ëR;¶G]:KYc`NÊB{ ür¥ôè
;6oå§0{a±ðÚÝU˜Ëµ0L¾Ë®Œ~`ã¯|—óªµx¬FOÆGÔbH¯VäQLõ—�7¨é†âudu[-È•…"K‚PWsñ_#–
= ¯ÎåÏý§yr³Ç°úzõF°¯{¦±9Øáj¨¶S!|"¥J%q½0ü7ZàÑ2SU÷R�úl+Þ1_ð�0sýÉv’†ß09×¿)IÁ‚~cb=”2	™<[Ÿ÷ó
ó8®-esiýæò²×5¬ex°ze@lóI�LÔs>â'¦(C]ä]µ+e§Ðhœä9Ña/GÆLÁóÕaùz>d®É.ùP85(žW>mvŸŽ@hÕñÐAzw›"Èœéq|ã¥� ¸†‡0].Y¹ä,ä7à^Ç(ð`Ì™¦v–Ù)ÙYñp
K•ˆYC�“}§jUCËÉ2 u[v´¸	<¬×îtr0ß‰àãQ¹j�5EÐ’<ÕLo bûaÌ”)U?·€´fz)l½^xHÚ;ÜØÞïÑáóåÅ©ž•çe¹çðŸ�šzm6y^I±¥‡�‘A×Û ":Y¼žÛƒ´øŽO!©º«¥}ˆ…Û|Íö&È(°éŸÑ©Šjc‡Ú�b*&¡Kè¦mc*jÚsJdÈä'8Æý…'>.,—�¼’!»Z3�Cš£<Å6¢–DÐû—ÓÅ¬ÊVã¨
7™!u½tÌ&'ÛôÌ\å•›:6¼èñ¶3€ƒ0¢Aÿ–(Rà&×ƒNŽ%Ë.µ�Ò»½â
Ž`e|)‡në‚#î¢Væ‘ÝpnÕÿp‹m’ˆóÞ¯yFAÖúƒ�$z¡;”ÛRz<HËÚPù)Ù¯ñ'¡Wá0I–T‰r¾<µ[žÁûÿª6ÚŸYž¨	Ð—^þ?6‚bŠØ4øó—h�Výž.�œÀ,�™§k7H¹# > nàê›û.¯´yûF)žÍ:b8.¥ØJU .>þ*OÇ¥É3Y/_ž�@Ê»Vë¯L¯>‹ª�|—\Ç¯‹_Ëª”—™L¨Z9Ki
\Bá¤¯µ3åöfnyÓ¨5–•ÕÜm¯3—l‰ ¿´÷k4�ÿ?'Ï÷ó”güs³¢*ºØ°¢Í®í·ŽN&`Xï¾TTÐÀ¯"ÈT'5ÌÜzû\êÀWµ=ÓúÆù“5i™ŒÖf£SÂõŒÖ¶»¡Š¾Ø!Ÿ:úz„Ða4Ø�áF� ÂY"ûHWiŸä†QŠLÞ.KÌu$F©ˆB§ý•³­_qÆé[—È¥DÕƒå™¹S–—ß1ÜZÁ¢· ý �Ëpê¬ÈõK\ÍÃ÷	Þ³ÏA±’ =Ô|=÷™(å*-çÖ“¿¿¼MmLé;ö›Éöà·íìm@êÝ#L˜r†Riáj9+•Å+ˆÉ9Í’Ö«¢8•Œ5Üò~£åO+ßÙÈ¯×p…–b,BÝ¦S¹«HJ·ÅoÛp¤ ô°†Æ\Æ¥MùKW'öÇ€Hv= «£ª=þ�ÈôâF\Bƒâ>ÇöÓU -“8éÓ¯ KE)/ß›?¥-Ó¿ÝrD
Ýw†L8þ_PÒõuD ™–Bh –EB�KŒépÉ¸jñ´ëÃ��ù.Zv†¥	d-ˆÚœ|Ôý¸›VB •…€ßLrÿ³z)MJù¾\µp™üÒÖÆ´=ÚñÞž¼àšj�–r‚«ló™îtß@¹Æ¹§¸[‡l õ©Zjì|ÄtÂŠØÆVþ°aaó.	'ªn¶%öÿ»hå"ømôû[£FÁõpbŽ>GÜK¬c:Ê7Zý(¼¦¬õêsÏËss+r¦NZëÝµ¶“l[à/óÉ˜—0ÙVR¯ˆ¿Aå^ûú¾l2í˜øF0®Ë´z(îoú2M˜.Û=€Œï0räŠÜ«T_0u‘¿¾"³ò#fv“L×†m'œÔ‚o¢vö0!�ò
_l ‡Uë]=Êî¦y8êDÃ0V…=5ì»/He³#±ãOvŠ½48çUÇ„*?ðåêæs.vž)ði"û…'ÁxÈ]q)Ç¶€È�ùËc8ªy;1P�r£äõ"©=iSèšºkñB�I¾Ï²+�ÎØž6¦œ�4é�ÎšÅÉ-à!âë:YE†¤–å¾ˆ”¡^’iXrœüÜµ
Ú¸éî
’‹§Y{x¹”Þ™M¬¤îRš­©l÷¶œ%ÛX@‹CH]a¾óïU¿vkdú–`�†:zzÌ3Gå=ÄÆÐ*4�œvPPŽîñ´ÃT*Iç‹>ýÒ6"Ää�’2·‰U8t(J4{ÔÔ±Ä*œ†îÍ»;VfÁÆíx¿4V(‡V·þSÌpâ‹P6h|3®{'²ËÒ#úÛ‡ëÆ\eùØÇg_j‡å‚È…v ÞûûL   tTl5‡ÄY½ ã‹Ã Ñ¤Ov±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  („<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1782" maxFusedRunOn="1782" detectionMode="exactProteinMatch"><Segment range="1753-1779" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1782" maxFusedRunOn="1782" detectionMode="exactProteinMatch"><Segment range="1723-1749" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1782" maxFusedRunOn="1782" detectionMode="exactProteinMatch"><Segment range="1693-1719" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4709-4725" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4685-4701" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4778-4799" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4733-4739" color="#ffffff" type="standard"/><Segment range="4740-4757" color="#ffffff" type="standard"/><Segment name="-35" range="4758-4763" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2457-2505" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6707-6811" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4515-4636" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3329-3464" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3149-3478" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-3135" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5087-5675" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1803-2391" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3545-4339" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="18" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6637" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5846-6637" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6638-6706" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="19" name="CREB1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1782" maxFusedRunOn="1782"><Segment range="706-1686" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>3438b123-5620-4f2c-9280-8984cddf2d3d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-CREB1(human)-3Ã—HA-WPRE-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="21:3:44">2025.9.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             	<StrandColors><TopStrand><ColorRange range="704..704" colors="green"/><ColorRange range="1686..1691" colors="green"/></TopStrand><BottomStrand><ColorRange range="704..704" colors="green"/><ColorRange range="1686..1691" colors="green"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
